Objective To investigate the relationship between intestinal tract normal bacteria flora and non-organic anorexia. Methods Stools of normal children and children with non-organic anorexia were cultured in aerobic or anaerobic environment respectively,and then the bacteria were identified and counted. Results There was significant difference in the number of Bifidobacterium Lactobacillus and intestinal flora between the two groups. Conclusion Close relation exists between non-organic anorexia and imbalance of intestinal normal flora.