Effects of fish meal replacement by soybean meal with supplementation of functional compound additives on intestinal morphology and microbiome of Japanese seabass ( Lateolabrax japonicus )

Aquaculture ResearchVolume 48, Issue 5 p. 2186-2197 Original Article Effects of fish meal replacement by soybean meal with supplementation of functional compound additives on intestinal morphology and microbiome of Japanese seabass (Lateolabrax japonicus) Jie Wang, Jie Wang The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorQingyan Tao, Qingyan Tao Novus International, Inc., St Louis, MO, USASearch for more papers by this authorZhen Wang, Zhen Wang The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorKangsen Mai, Kangsen Mai The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorWei Xu, Wei Xu The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorYanjiao Zhang, Yanjiao Zhang The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorQinghui Ai, Corresponding Author Qinghui Ai qhai@ouc.edu.cn The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaCorrespondence: Q Ai, Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China. E-mail: qhai@ouc.edu.cnSearch for more papers by this author Jie Wang, Jie Wang The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorQingyan Tao, Qingyan Tao Novus International, Inc., St Louis, MO, USASearch for more papers by this authorZhen Wang, Zhen Wang The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorKangsen Mai, Kangsen Mai The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorWei Xu, Wei Xu The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorYanjiao Zhang, Yanjiao Zhang The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaSearch for more papers by this authorQinghui Ai, Corresponding Author Qinghui Ai qhai@ouc.edu.cn The Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, Qingdao, Shandong, ChinaCorrespondence: Q Ai, Key Laboratory of Aquaculture Nutrition and Feed (Ministry of Agriculture) and Key Laboratory of Mariculture (Ministry of Education), Ocean University of China, 5 Yushan Road, Qingdao, Shandong 266003, China. E-mail: qhai@ouc.edu.cnSearch for more papers by this author First published: 16 April 2016 https://doi.org/10.1111/are.13055Citations: 54Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract A 15-week trial was carried out to estimate the effects of functional compound additives (FCA) on intestinal morphology and microbiome in Japanese seabass, Lateolabrax japonicus, fed diets with soybean meal (SBM) partially replacing fish meal (FM). The formulation of FCA was the mixture of antioxidant, immunopotentiator and Mintrex® trace elements. Four isonitrogenous (45%) and isolipidic (11%) diets, including FM42 (the control group without FCA), FM35 (15% FM protein substitution level with FCA), FM21 (50% FM protein substitution level with FCA) and FM21-N (50% FM protein substitution level without FCA) were formulated. Quadruplicate groups of seabass (initial average weight 125.65 ± 0.60 g) were randomly handfed each diet twice daily in seawater floating cage. No significant differences were found in microvillus height (HMV) and muscular thickness (MT) of distal intestine among fish fed FM42, FM35 and FM21, whereas the HMV, MT and fold height (HF) in seabass fed FM42 were significantly higher than those of seabass fed FM21-N (P < 0.05). Seabass fed FM21-N had more obvious enteritis parameters such as tissue disruption, wider lamina propria and less mucosal fold than those in seabass fed FM21. High-throughput sequencing technology of gut flora showed that Proteobacteria was the most dominant bacteria at phylum level among groups. There was no significant difference in relative abundance and composition of gut microflora among groups. In conclusion, supplementation of FCA might partially eliminate SBM-induced enteritis, but the composition and relative abundance of intestinal microflora were not affected by dietary treatments. Citing Literature Volume48, Issue5May 2017Pages 2186-2197 RelatedInformation