Dihydromyricetin alleviates carbon tetrachloride-induced acute liver injuryviaJNK-dependent mechanism in mice

AIM:To assess the effects of dihydromyricetin (DHM) as a hepatoprotective candidate in reducing hepatic injury and accelerating hepatocyte proliferation after carbon tetrachloride (CCl4) treatment.METHODS: C57 BL/6 mice were used in this study.Mice were orally administered with DHM (150 mg/kg) for 4 d after CCl4 treatment.Serum and liver tissue samples were collected on days 1, 2, 3, 5 and 7 after CCl4 treatment.The anti-inflammatory effect of DHM was assessed directly by hepatic histology detection and indirectly by serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), albumin, and superoxide dismutase (SOD).Inflammatory cytokines, such as interleukin (IL)-1β, IL-6 and tumor necrosis factor-α (TNF-α), were detected using ELISA kits.Proliferating cell nuclear antigen (PCNA) staining was used to evaluate the role of DHM in promoting hepatocyte proliferation.Hepatocyte apoptosis was