[A method of acutely isolating rat dorsal root ganglion neurons for patch-clamp study of single-channel].

To establish a method of acutely isolating dorsal root ganglion (DRG) neurons for patch clamp study of single-channel.DRG neurons of rats were acutely isolated by enzymatic digestion and mechanical blowing.The acutely isolated DRG cells were easy to form the higher sealing resistance (> 5G Omega), which lowered noise level, so that pA-level single channel currents could be recorded.The acutely isolated DRG neurons in this study are an ideal for patch-clamp study of single-channel.