A Comparison of Routine Culture with Polymerase Chain Reaction Technology for the Detection of Mycoplasma Species in Feline Nasal Samples

支原体 聚合酶链反应 生物 微生物培养 活检 微生物学 软体动物 病理 医学 细菌 内科学 遗传学 基因 生物化学
作者
Lynelle R. Johnson,Nicole L. Drazenovich,Janet E. Foley
出处
期刊:Journal of Veterinary Diagnostic Investigation [SAGE Publishing]
卷期号:16 (4): 347-351 被引量:30
标识
DOI:10.1177/104063870401600418
摘要

Nasal flush samples were collected from 20 cats and submitted for Mycoplasma culture and polymerase chain reaction (PCR). Nasal biopsy samples were also obtained from each cat and simultaneously evaluated for Mycoplasma by standard culture and PCR. Concordance of the test results was determined through calculation of the kappa statistic. In 6 cats, nasal flush samples were culture positive for Mycoplasma. PCR was positive in each culture-positive cat and also positive in 1 flush sample that was culture negative. DNA sequencing of the PCR product from the culture negative flush sample identified the organism as Mycoplasma arginini. All other flush samples that were culture negative were also PCR negative (kappa = 0.89). Nasal biopsy samples from 7 cats were culture positive for Mycoplasma, and all were PCR positive. Biopsy samples that were culture negative for Mycoplasma were also PCR negative (kappa = 1.0). Results of culture and PCR for both nasal flush and biopsy were concordant in 19 of 20 cats, and PCR was able to identify an unusual Mycoplasma species that did not grow in culture. In most cats, organisms could be detected in either nasal flush or biopsy samples. In this study, PCR provided rapid and sensitive detection of Mycoplasma species in nasal samples from cats and detected 1 organism that did not grow in culture.
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