Phage Display : A Laboratory Manual

SECTION 1 - PHAGE DISPLAY Chapter 1. Filamentous Phage Biology Chapter 2. Phage-display Vectors Chapter 3. Antibody Libraries Chapter 4. Peptide Libraries Chapter 5. Functional Domains and Scaffolds Chapter 6. Gene Fragment Libraries and Genomic and cDNA Expression Cloning SECTION 2 - ANTIBODY LIBRARIES Chapter 7. Overview: Amplification of Antibody Genes Chapter 8. Generation of Antibody Libraries: Immunization, RNA Preparation, and cDNA Synthesis Chapter 9. Generation of Antibody Libraries: PCR Amplification and Assembly of Light- And Heavy-chain Coding Sequences Chapter 10. Selection from Antibody Libraries Chapter 11. Analysis of Selected Antibodies Chapter 12. Production and Purification of Fab and scFv Chapter 13. Antibody Engineering SECTION 3 - PEPTIDE LIBRARIES Chapter 14. Overview: Peptide Libraries Chapter 15. General Phage Methods Chapter 16. Production of Peptide Libraries Chapter 17. Screening Peptide Libraries Chapter 18. Analysis of Phage-borne Peptides Chapter 19. Construction and Use of pIII-displayed Peptide Libraries SECTION 4 - OTHER LIBRARIES AND OTHER METHODS OF PANNING Chapter 20. Construction and Selection from Gene Fragment Phage-display Expression Libraries Chapter 21. Construction and Selection from cDNA Phage-display Expression Libraries Chapter 22. In Vivo Selection of Phage-display Libraries Chapter 23. Cell-surface Selection and Analysis of Monoclonal Antibodies from Phage Libraries SECTION 5 - APPENDICES Appendix 1. Useful Information Appendix 2. Recipes Appendix 3. General Procedures Appendix 4. Cautions Appendix 5. Suppliers Appendix 6. Trademarks