蟾蜍
毒液
细胞毒性
体外
化学
MTT法
细胞培养
高效液相色谱法
色谱法
立体化学
生物化学
生物
生态学
遗传学
作者
Hui‐Min Gao,Martin Zehl,Hanspeter Kaehlig,P. Schneider,Hermann Stuppner,Laetitia Moreno Y Banuls,Róbert Kiss,Brigitte Kopp
摘要
Toad venom, namely, “Chansu” in China, has been widely used for the treatment of heart failure, sores, pains, and various cancers. Upon LC-MS analysis of the venom from Bufo melanosticus collected in Indonesia, new bufadienolide sulfates were identified. For a complete characterization, the MeOH extract of the toad venom from B. melanosticus was fractionated by preparative HPLC, and the structures of five new buadienolide sulfates (1−5) along with one new bufogenin (6) were rapidly elucidated on the basis of LC-DAD-MSn and LC-SPE-NMR data. The in vitro growth inhibitory activity of these six compounds along with hellebrin (positive control) has been assayed by means of the MTT colorimetric assay in four human and two mouse cancer cell lines. Compound 3 and hellebrin displayed similar and marked in vitro cytotoxicity.
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