表位
病毒学
生物
抗原
保守序列
肽序列
人类免疫缺陷病毒(HIV)
计算生物学
遗传学
基因
作者
Will Fischer,Simon Perkins,James Theiler,Tanmoy Bhattacharya,Karina Yusim,Robert Funkhouser,Carla Kuiken,Barton F. Haynes,Norman L. Letvin,Bruce D. Walker,Beatrice H. Hahn,Bette Korber
出处
期刊:Nature Medicine
[Springer Nature]
日期:2006-12-24
卷期号:13 (1): 100-106
被引量:432
摘要
HIV-1/AIDS vaccines must address the extreme diversity of HIV-1. We have designed new polyvalent vaccine antigens comprised of sets of 'mosaic' proteins, assembled from fragments of natural sequences via a computational optimization method. Mosaic proteins resemble natural proteins, and a mosaic set maximizes the coverage of potential T-cell epitopes (peptides of nine amino acids) for a viral population. We found that coverage of viral diversity using mosaics was greatly increased compared to coverage by natural-sequence vaccine candidates, for both variable and conserved proteins; for conserved HIV-1 proteins, global coverage may be feasible. For example, four mosaic proteins perfectly matched 74% of 9-amino-acid potential epitopes in global Gag sequences; 87% of potential epitopes matched at least 8 of 9 positions. In contrast, a single natural Gag protein covered only 37% (9 of 9) and 67% (8 of 9). Mosaics provide diversity coverage comparable to that afforded by thousands of separate peptides, but, because the fragments of natural proteins are compressed into a small number of native-like proteins, they are tractable for vaccines.
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