突触小泡
快照25
囊泡融合
小泡
突触蛋白1
接吻奔跑融合
活动区
细胞生物学
蒙克-18
生物
生物物理学
化学
对接(动物)
神经科学
生物化学
膜
医学
护理部
作者
Cordelia Imig,Sangwon Min,Stefanie Krinner,Marife Arancillo,Christian Rosenmund,Thomas C. Südhof,JeongSeop Rhee,Nils Brose,Benjamin H. Cooper
出处
期刊:Neuron
[Elsevier]
日期:2014-10-01
卷期号:84 (2): 416-431
被引量:328
标识
DOI:10.1016/j.neuron.2014.10.009
摘要
Synaptic vesicle docking, priming, and fusion at active zones are orchestrated by a complex molecular machinery. We employed hippocampal organotypic slice cultures from mice lacking key presynaptic proteins, cryofixation, and three-dimensional electron tomography to study the mechanism of synaptic vesicle docking in the same experimental setting, with high precision, and in a near-native state. We dissected previously indistinguishable, sequential steps in synaptic vesicle active zone recruitment (tethering) and membrane attachment (docking) and found that vesicle docking requires Munc13/CAPS family priming proteins and all three neuronal SNAREs, but not Synaptotagmin-1 or Complexins. Our data indicate that membrane-attached vesicles comprise the readily releasable pool of fusion-competent vesicles and that synaptic vesicle docking, priming, and trans-SNARE complex assembly are the respective morphological, functional, and molecular manifestations of the same process, which operates downstream of vesicle tethering by active zone components.
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