卵黄周隙
透明带
卵母细胞
人类受精
卵胞浆内精子注射
男科
合子
生物
胚胎
极体
胚胎质量
体外受精
胚胎移植
解剖
精子
胚胎发生
医学
细胞生物学
作者
Maria das Graças R. S. Camargos,Veronica N. M. Lobach,Francisco de Assis Nunes Pereira,Cláudia Lemos,Fernando M. Reis,Aroldo F. Camargos
标识
DOI:10.1177/1933719111420884
摘要
The present study aimed to correlate morphometric parameters of the oocytes with the occurrence of fertilization following intracytoplasmic sperm injection (ICSI). In a prospective, controlled cohort design, women (n = 32) who were candidates for ICSI had oocytes (n = 258) collected and submitted to morphometric evaluation using the Cronus3 software program. The morphometric parameters obtained were oocyte diameter, perivitelline space width, zona pellucida thickness, and first polar body diameter. The median oocyte diameter was similar in cases in which fertilization occurred compared with those in which fertilization failed (75.2 and 75.9 μm, respectively; P = .218). The 2 groups also had similar measurements of perivitelline space, zona pellucida, and first polar body. However, the best quality zygotes identified by a morphological score resulted from oocytes with larger diameter (75.6 vs 74.0 μm; P < .01) and narrow perivitelline space (5.3 vs 7.1 μm; P < .01). Embryo development, as assessed by cleavage at second day of culture, was not significantly associated with oocyte morphometric parameters. These findings suggest that morphometric parameters of the oocytes do not correlate with the occurrence of fertilization following ICSI but may assist in selecting oocytes more likely to originate high-quality zygotes.
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