生发中心
生物
流式细胞术
荧光显微镜
B细胞
分子生物学
绿色荧光蛋白
荧光
细胞生物学
亲和力成熟
抗原
抗体
遗传学
物理
光学
基因
作者
Gabriel D. Victora,Tanja Schwickert,David Fooksman,Alice O. Kamphorst,Michael Meyer–Hermann,Michael L. Dustin,Michel C. Nussenzweig
出处
期刊:Cell
[Elsevier]
日期:2010-11-01
卷期号:143 (4): 592-605
被引量:1037
标识
DOI:10.1016/j.cell.2010.10.032
摘要
The germinal center (GC) reaction produces high-affinity antibodies by random mutation and selective clonal expansion of B cells with high-affinity receptors. The mechanism by which B cells are selected remains unclear, as does the role of the two anatomically defined areas of the GC, light zone (LZ) and dark zone (DZ). We combined a transgenic photoactivatable fluorescent protein tracer with multiphoton laser-scanning microscopy and flow cytometry to examine anatomically defined LZ and DZ B cells and GC selection. We find that B cell division is restricted to the DZ, with a net vector of B cell movement from the DZ to the LZ. The decision to return to the DZ and undergo clonal expansion is controlled by T helper cells in the GC LZ, which discern between LZ B cells based on the amount of antigen captured and presented. Thus, T cell help, and not direct competition for antigen, is the limiting factor in GC selection.
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