Sublines of mouse 3T3 cells that accumulate lipid

From the established mouse fibroblast line 3T3, we have isolated two clonal sublines that accumulate large amounts of triglyceride fat when the cells are in the resting state. The accumulation is reduced by lipolytic agents. Unless lipid accumulation is allowed to proceed too far, the fatty cells begin to grow again when they are transferred and in this way eliminate most of their lipid.