隐色素
昼夜节律
视交叉上核
生物
句号(音乐)
生物钟
内分泌学
内科学
黑暗
光对昼夜节律的影响
突变体
时钟
细胞生物学
基因
遗传学
植物
医学
物理
声学
作者
Martha Hotz Vitaterna,Christopher P. Selby,Takeshi Todo,Hitoshi Niwa,Carol L. Thompson,Ethan M. Fruechte,Katsundo HITOMI,Randy Thresher,Tomoko Ishikawa,Jun‐ichi Miyazaki,Joseph S. Takahashi,Aziz Sancar
标识
DOI:10.1073/pnas.96.21.12114
摘要
Cryptochromes regulate the circadian clock in animals and plants. Humans and mice have two cryptochrome ( Cry ) genes. A previous study showed that mice lacking the Cry2 gene had reduced sensitivity to acute light induction of the circadian gene mPer1 in the suprachiasmatic nucleus (SCN) and had an intrinsic period 1 hr longer than normal. In this study, Cry1 −/− and Cry1 −/− Cry2 −/− mice were generated and their circadian clocks were analyzed at behavioral and molecular levels. Behaviorally, the Cry1 −/− mice had a circadian period 1 hr shorter than wild type and the Cry1 −/− Cry2 −/− mice were arrhythmic in constant darkness (DD). Biochemically, acute light induction of mPer1 mRNA in the SCN was blunted in Cry1 −/− and abolished in Cry1 −/− Cry2 −/− mice. In contrast, the acute light induction of mPer2 in the SCN was intact in Cry1 −/− and Cry1 −/− Cry2 −/− animals. Importantly, in double mutants, mPer1 expression was constitutively elevated and no rhythmicity was detected in either 12-hr light/12-hr dark or DD, whereas mPer2 expression appeared rhythmic in 12-hr light/12-hr dark, but nonrhythmic in DD with intermediate levels. These results demonstrate that Cry1 and Cry2 are required for the normal expression of circadian behavioral rhythms, as well as circadian rhythms of mPer1 and mPer2 in the SCN. The differential regulation of mPer1 and mPer2 by light in Cry double mutants reveals a surprising complexity in the role of cryptochromes in mammals.
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