CmSCL4 and CmR1MYB1 synergistically enhance the drought tolerance by regulation of ABA signaling in chrysanthemum

拟南芥 生物 耐旱性 转录因子 MYB公司 脱落酸 细胞生物学 异源表达 异源的 异位表达 转基因作物 基因 转基因 拟南芥 遗传学 植物 突变体 重组DNA
作者
Ting Zhang,Yixin Qu,Haibin Wang,Zhenxing Wang,Jiafu Jiang,Sumei Chen,Weimin Fang,Zhiyong Guan,Yuan Liao,Fadi Chen
出处
期刊:Environmental and Experimental Botany [Elsevier BV]
卷期号:199: 104886-104886 被引量:4
标识
DOI:10.1016/j.envexpbot.2022.104886
摘要

With water shortage worldwide, seeking drought resistance genes are of great value for molecular breeding. Here, we isolated a homologous AtSCL4/7 subfamily gene of GRAS transcription factor family from chrysanthemum and designated it as CmSCL4. CmSCL4 was responded to 20% PEG6000 treatment and its heterologous expression improved drought tolerance by up-regulating ABA signaling genes AtABI3 and AtABI4 in Arabidopsis. Moreover, the cotyledon greening rate, early seedling development and stomatal aperture of transgenic Arabidopsis are hypersensitivity to external ABA treatment. Meanwhile, overexpression and chimeric repression of CmSCL4 in chrysanthemum also conferred the increased or decreased drought tolerance and corresponding changes of the expression of CmABI4. Yeast two-hybrid screening of the cDNA library of chrysanthemum by CmSCL4 identified a MYB transcription factor as an important candidate partner, which was named as CmR1MYB1 hereafter. The interaction between CmSCL4 and CmR1MYB1 was further verified by in vitro pull-down, in vivo BiFC and Co-IP assays. CmR1MYB1 and CmSCL4 had similar expression patterns under 20% PEG6000 treatment. Heterologous expression of CmR1MYB1 in Arabidopsis also facilitated the expression levels of AtABI3 and AtABI4, resulted in the improved drought tolerance. These results demonstrated that CmSCL4 cooperates with CmR1MYB1 to modulate the dehydration tolerance of chrysanthemum, at least partially through the regulation of ABA-responsive genes.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
深情安青应助Kair采纳,获得10
3秒前
8秒前
科研通AI2S应助郑恩熙采纳,获得10
13秒前
陈皮完成签到 ,获得积分10
16秒前
Singularity应助科研通管家采纳,获得10
20秒前
20秒前
田様应助科研通管家采纳,获得10
20秒前
劲秉应助科研通管家采纳,获得10
20秒前
劲秉应助科研通管家采纳,获得10
20秒前
ccc应助科研通管家采纳,获得10
20秒前
星辰大海应助科研通管家采纳,获得10
20秒前
我是老大应助科研通管家采纳,获得10
20秒前
科研通AI2S应助科研通管家采纳,获得10
20秒前
Singularity应助科研通管家采纳,获得10
20秒前
YingyingFan应助科研通管家采纳,获得10
20秒前
Singularity应助科研通管家采纳,获得10
20秒前
热心路人应助科研通管家采纳,获得10
21秒前
劲秉应助科研通管家采纳,获得10
21秒前
Singularity应助科研通管家采纳,获得10
21秒前
科研通AI2S应助科研通管家采纳,获得10
21秒前
ccc应助科研通管家采纳,获得10
21秒前
Singularity应助科研通管家采纳,获得10
21秒前
Singularity应助科研通管家采纳,获得10
21秒前
YingyingFan应助科研通管家采纳,获得10
21秒前
Singularity应助科研通管家采纳,获得10
21秒前
ccc应助科研通管家采纳,获得10
22秒前
tuanheqi应助科研通管家采纳,获得150
22秒前
林利芳完成签到 ,获得积分10
32秒前
32秒前
落雪慕卿颜完成签到,获得积分10
33秒前
追逐123完成签到 ,获得积分10
34秒前
40秒前
郑恩熙完成签到,获得积分10
42秒前
shenglll完成签到 ,获得积分10
42秒前
微笑芒果完成签到 ,获得积分10
45秒前
友好冥王星完成签到 ,获得积分10
48秒前
gao完成签到 ,获得积分10
56秒前
怡然猎豹完成签到,获得积分10
1分钟前
只鱼完成签到 ,获得积分10
1分钟前
lyj完成签到 ,获得积分10
1分钟前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2700
Neuromuscular and Electrodiagnostic Medicine Board Review 1000
こんなに痛いのにどうして「なんでもない」と医者にいわれてしまうのでしょうか 510
いちばんやさしい生化学 500
Genre and Graduate-Level Research Writing 500
The First Nuclear Era: The Life and Times of a Technological Fixer 500
岡本唐貴自伝的回想画集 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3674546
求助须知:如何正确求助?哪些是违规求助? 3229838
关于积分的说明 9787162
捐赠科研通 2940432
什么是DOI,文献DOI怎么找? 1611923
邀请新用户注册赠送积分活动 761063
科研通“疑难数据库(出版商)”最低求助积分说明 736488