Preparation of Metformin/Doxorubicin-Bovine Serum Albumin-Hyaluronic Acid-Carbon Dots Nanoparticles and Their Application in Treating Polycystic Ovary Syndrome on Matrix Metalloproteinase-9 and Vascular Endothelial Growth Factor of Patient Combine with Metformin

At present, carbon dot nanoparticle has good light stability and bioavailability, which has become a novel type of nano material widely employed in medicine. This investigation aimed to study the effects of nanomedicine based on Metformin/doxorubicin-bovine serum albumin-hyaluronic acid-carbon dots (Met/DOX-BSA-HA-CD) on matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF) of polycystic ovarian syndrome (PCOS) patients. First, Met/DOX-BSA-HA-CD was prepared from Met and doxorubicin (DOX) and BSA-HACD solution. 30 PCOS patients accepted by hospital were deemed as experimental group, and 30 healthy married women were taken as controls. Met/DOX-BSA-HA-CD nanomedicine was prepared and applied to the treatment of 30 PCOS patients. The results showed that the average particle size of Met/DOX-BSA-HA-CD was about 120 nm. MMP-9 immunohistochemical H-Score (285.32±36.41 points) of the endometrial glandular epithelium of experimental group was considerably inferior to controls (364.35±26.74 points) ( P < 0.05). VEGF level of experimental group (103.25±20.64 pg/mL) was substantially superior to controls (86.32±14.21 pg/mL) ( P < 0.05). Moreover, serum VEGF level was positively correlated with body mass index ( r = 0.236, P < 0.05), and negatively correlated with follicle stimulating hormone/luteinizing hormone (FSH/LH) ( r =−0.061, P < 0.05). The carbon dots prepared in this study had good light stability. The ratio of nanocarrier to drug: bovine serum albumin-hyaluronic acid-carbon dots:metformin = 4:1 was determined as the feeding ratio, and the embedding rate was 39.8%. The expression of MMP-9 in the endometrial glandular epithelium of PCOS patients was expected to be an indicator of endometrial receptivity. PCOS patients had endocrine disorders and abnormal VEGF levels.