清脆的
生物
计算生物学
小RNA
基因
基因组编辑
生物发生
功能(生物学)
遗传学
Cas9
作者
Fenglin Deng,Fanrong Zeng,Qiufang Shen,Asad Abbas,Jianhui Cheng,Wei Jiang,Guang Chen,Adnan Noor Shah,Paul Holford,Mohsin Tanveer,Dabing Zhang,Zhong‐Hua Chen
标识
DOI:10.1016/j.tplants.2022.01.009
摘要
Gene editing using clustered regularly interspaced short palindromic repeat/CRISPR-associated proteins (CRISPR/Cas) has revolutionized biotechnology and provides genetic tools for medicine and life sciences. However, the application of this technology to miRNAs, with the function as negative gene regulators, has not been extensively reviewed in plants. Here, we summarize the evolution, biogenesis, and structure of miRNAs, as well as their interactions with mRNAs and computational models for predicting target genes. In addition, we review current advances in CRISPR/Cas for functional analysis and for modulating miRNA genes in plants. Extending our knowledge of miRNAs and their manipulation with CRISPR will provide fundamental understanding of the functions of plant miRNAs and facilitate more sustainable and publicly acceptable genetic engineering of crops.
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