Finding intracellular lipid droplets from the single-cell biolens’ signature in a holographic flow-cytometry assay

流式细胞术 细胞仪 细胞内 显微镜 病理 医学 生物 细胞生物学 分子生物学
作者
Daniele Pirone,Daniele Sirico,Martina Mugnano,Danila del Giudice,Ivana Kurelac,Beatrice Cavina,Pasquale Memmolo,Lisa Miccio,Pietro Ferraro
出处
期刊:Biomedical Optics Express [The Optical Society]
卷期号:13 (11): 5585-5585 被引量:6
标识
DOI:10.1364/boe.460204
摘要

In recent years, intracellular LDs have been discovered to play an important role in several pathologies. Therefore, detection of LDs would provide an in-demand diagnostic tool if coupled with flow-cytometry to give significant statistical analysis and especially if the diagnosis is made in full non-invasive mode. Here we combine the experimental results of in-flow tomographic phase microscopy with a suited numerical simulation to demonstrate that intracellular LDs can be easily detected through a label-free approach based on the direct analysis of the 2D quantitative phase maps recorded by a holographic flow cytometer. In fact, we demonstrate that the presence of LDs affects the optical focusing lensing features of the embracing cell, which can be considered a biological lens. The research was conducted on white blood cells (i.e., lymphocytes and monocytes) and ovarian cancer cells. Results show that the biolens properties of cells can be a rapid biomarker that aids in boosting the diagnosis of LDs-related pathologies by means of the holographic flow-cytometry assay for fast, non-destructive, and high-throughput screening of statistically significant number of cells.

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