神经突
神经保护
化学
脊髓损伤
细胞生物学
再生(生物学)
间充质干细胞
姜黄素
星形胶质细胞
小胶质细胞
神经干细胞
药理学
脊髓
干细胞
免疫学
炎症
体外
生物化学
中枢神经系统
生物
神经科学
作者
Xiang Gao,Zhihui Han,Cheng Huang,Huali Lei,Guangqiang Li,Lin Chen,Feng Dandan,Zijie Zhou,Qin Shi,Liang Cheng,Xiaozhong Zhou
标识
DOI:10.1016/j.bioactmat.2022.05.026
摘要
Spinal cord regeneration after a spinal cord injury (SCI) remains a difficult challenge due to the complicated inflammatory microenvironment and neuronal damage at the injury sites. In this study, retinoic acid (RA) and curcumin (Cur) were co-loaded with bovine serum albumin (BSA) self-assembly to obtain RA@BSA@Cur nanoparticles (NPs) for SCI treatment. Cur, as an antioxidant drug, facilitated reactive oxygen species (ROS) scavenging, and decreased the amount of inflammatory factors secreted by macrophages, while RA could enhance neurite extensions and neural differentiation. The constructed RA@BSA@Cur NPs not only induced polarization of macrophages toward pro-regenerative phenotypes and markedly reduced the inflammatory response of macrophages or microglia, but also increased neurite length in PC12 cells and neuronal differentiation of bone marrow mesenchymal stem cells, improved the differentiation of neural stem cells (NSCs) into β3-tubulin+ neurons, and reversed the pro-astrocyte differentiation effect of inflammatory cytokines on NSCs. In vivo experiments revealed that RA@BSA@Cur NPs regulated the phenotypic polarization of macrophages, inhibited the release of inflammatory mediators, promoted functional neuron regeneration and motor function, and further inhibited scar tissue formation. This study highlighted that the BSA-based biomimetic nanomaterials could be used as ROS scavengers and nerve regeneration promoters for treating SCI.
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