生物
器官发生
间充质干细胞
薄壁组织
Wnt信号通路
原位杂交
细胞生物学
核糖核酸
肺
细胞
胚胎干细胞
信号转导
遗传学
基因表达
内科学
基因
医学
植物
作者
Nicholas M. Negretti,Erin J. Plosa,John T. Benjamin,Bryce Schuler,Arun C. Habermann,Christopher S. Jetter,Peter M. Gulleman,Claire Bunn,Alice N. Hackett,Meaghan Ransom,Chase J. Taylor,David S. Nichols,Brittany K. Matlock,Susan H. Guttentag,Timothy S. Blackwell,Nicholas E. Banovich,Jonathan A. Kropski,Jennifer M. S. Sucre
出处
期刊:Development
[The Company of Biologists]
日期:2021-12-15
卷期号:148 (24)
被引量:83
摘要
ABSTRACT Lung organogenesis requires precise timing and coordination to effect spatial organization and function of the parenchymal cells. To provide a systematic broad-based view of the mechanisms governing the dynamic alterations in parenchymal cells over crucial periods of development, we performed a single-cell RNA-sequencing time-series yielding 102,571 epithelial, endothelial and mesenchymal cells across nine time points from embryonic day 12 to postnatal day 14 in mice. Combining computational fate-likelihood prediction with RNA in situ hybridization and immunofluorescence, we explore lineage relationships during the saccular to alveolar stage transition. The utility of this publicly searchable atlas resource (www.sucrelab.org/lungcells) is exemplified by discoveries of the complexity of type 1 pneumocyte function and characterization of mesenchymal Wnt expression patterns during the saccular and alveolar stages – wherein major expansion of the gas-exchange surface occurs. We provide an integrated view of cellular dynamics in epithelial, endothelial and mesenchymal cell populations during lung organogenesis.
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