缺氧(环境)
化学
生物医学中的光声成像
硝基还原酶
纳米颗粒
纳米技术
肿瘤缺氧
胶体金
材料科学
生物物理学
氧气
医学
光学
生物化学
内科学
酶
放射治疗
生物
物理
有机化学
作者
Xin Li,Xueyang Fang,Shiying Li,Kwok-Ho Lui,Wai‐Sum Lo,Yanjuan Gu,Wing‐Tak Wong
标识
DOI:10.1166/jbn.2021.3195
摘要
Hypoxia is an important phenomenon due to insufficient oxygen supply in tumor tissue, and nitroreductase (NTR) is a characteristic enzyme used for evaluating hypoxia level in tumors. In this work, we designed a smart gold nanoparticle (AuNPs), modified by 16-mercaptoundecanoic acid (MHDA) and hypoxia-responsive 11-(2-nitro-1H-imidazol-1-yl)undecane-1-thiol (NI) ligand, that responds to the hypoxic environment in tumor sites. With proper surface ligand composition, the responsive nanoprobe exhibited aggregation through the bioreduction of the nitro group on NI ligands under hypoxic conditions and the UV-vis absorption peak maximum would shift to 630 nm from 530 nm, which acts as an "off-on" contrast agent for tumor hypoxic photoacoustic (PA) imaging. In vitro and in vivo experiments revealed that AuNPs@MHDA/NO₂ exhibited an enhanced PA signal in hypoxic conditions. This study demonstrates the potential of hypoxia-responsive AuNPs as novel and sensitive diagnostic agents, which lays a firm foundation for precise cancer treatment in the future.
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