磷酸化
泛素连接酶
基因沉默
基因敲除
泛素
癌症研究
SMAD公司
胶质瘤
生物
表型
突变体
干细胞
R-SMAD
细胞生物学
遗传学
细胞培养
基因
内皮糖蛋白
川地34
作者
Manami Hiraiwa,Kazuya Fukasawa,Takashi Iezaki,Hemragul Sabit,Tetsuhiro Horie,Kazuya Tokumura,Sayuki Iwahashi,Misato Murata,Masaki Kobayashi,Akane Suzuki,Gyujin Park,Katsuyuki Kaneda,Tomoki Todo,Atsushi Hirao,Mitsutoshi Nakada,Eiichi Hinoi
标识
DOI:10.1038/s42003-021-02950-0
摘要
Abstract Glioma stem cells (GSCs) contribute to the pathogenesis of glioblastoma, the most malignant form of glioma. The implication and underlying mechanisms of SMAD specific E3 ubiquitin protein ligase 2 (SMURF2) on the GSC phenotypes remain unknown. We previously demonstrated that SMURF2 phosphorylation at Thr 249 (SMURF2 Thr249 ) activates its E3 ubiquitin ligase activity. Here, we demonstrate that SMURF2 Thr249 phosphorylation plays an essential role in maintaining GSC stemness and tumorigenicity. SMURF2 silencing augmented the self-renewal potential and tumorigenicity of patient-derived GSCs. The SMURF2 Thr249 phosphorylation level was low in human glioblastoma pathology specimens. Introduction of the SMURF2 T249A mutant resulted in increased stemness and tumorigenicity of GSCs, recapitulating the SMURF2 silencing. Moreover, the inactivation of SMURF2 Thr249 phosphorylation increases TGF-β receptor (TGFBR) protein stability. Indeed, TGFBR1 knockdown markedly counteracted the GSC phenotypes by SMURF2 T249A mutant. These findings highlight the importance of SMURF2 Thr249 phosphorylation in maintaining GSC phenotypes, thereby demonstrating a potential target for GSC-directed therapy.
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