单核细胞增生李斯特菌
沙门氏菌
多路复用
李斯特菌
生物
再现性
微生物学
流式细胞术
细菌
色谱法
食品科学
化学
分子生物学
生物信息学
遗传学
作者
Qian Xu,Siyuan Liu,Shunshi Ji,Ziquan Wang,Meng Wang,Yingying Liu,Xiaoyun Gong,Boqiang Fu,Changyun Ye,Haiyan Chang,Zhiwei Sui
标识
DOI:10.1016/j.lwt.2022.113487
摘要
Chicken breast is prone to Listeria monocytogenes, Salmonella spp., and E. coli O157:H7 contaminations. Therefore, a quantitative method for the rapid detection of these pathogens simultaneously in chicken breast is highly desirable for food safety control. This study reports a flow cytometry (FCM)-based method for rapid and multiplexed quantification of the above-stated three pathogens without enrichment. Using the developed method, the complete detection process, including a sample pretreatment procedure, the multiplex staining, and the FCM analysis, could be realized within 1 h, rendering this method more efficient than the plate counting method. The bacterial recovery rates were 96.24% for Listeria monocytogenes, 96.74% for Salmonella, and 96.42% for E. coli O157:H7. A good linear correlation was realized between the FCM-based method and the plate counting method for all three pathogens. The relative standard deviations were 3.0%, 2.7%, and 2.9% for Listeria monocytogenes, Salmonella, and E. coli O157:H7, which indicated excellent reproducibility of the method. This study is the first one to report the rapid and simultaneous quantification of foodborne pathogens in chicken breast using FCM. Moreover, the developed method was also applied successfully to the multiplexed detection of bacteria, which indicated that the method has great potential in food contamination monitoring.
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