克隆(Java方法)
髓母细胞瘤
癌症干细胞
干细胞
神经干细胞
癌症研究
生物
癌症
脑瘤
人脑
病理
医学
细胞生物学
神经科学
遗传学
基因
作者
Sheila K. Singh,Ian D. Clarke,Mizuhiko Terasaki,Victoria E. Bonn,Cynthia Hawkins,Jeremy A. Squire,Peter B. Dirks
出处
期刊:PubMed
日期:2003-09-15
卷期号:63 (18): 5821-8
被引量:2660
摘要
Most current research on human brain tumors is focused on the molecular and cellular analysis of the bulk tumor mass. However, there is overwhelming evidence in some malignancies that the tumor clone is heterogeneous with respect to proliferation and differentiation. In human leukemia, the tumor clone is organized as a hierarchy that originates from rare leukemic stem cells that possess extensive proliferative and self-renewal potential, and are responsible for maintaining the tumor clone. We report here the identification and purification of a cancer stem cell from human brain tumors of different phenotypes that possesses a marked capacity for proliferation, self-renewal, and differentiation. The increased self-renewal capacity of the brain tumor stem cell (BTSC) was highest from the most aggressive clinical samples of medulloblastoma compared with low-grade gliomas. The BTSC was exclusively isolated with the cell fraction expressing the neural stem cell surface marker CD133. These CD133+ cells could differentiate in culture into tumor cells that phenotypically resembled the tumor from the patient. The identification of a BTSC provides a powerful tool to investigate the tumorigenic process in the central nervous system and to develop therapies targeted to the BTSC.
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