This study was performed to evaluate herbicidal activities of 17 natural compounds from plant sources. Lemna paucicostata and an alga, Chlorella regularis were used for initial screening for the in vitro herbicidal activity. In addition, the in vivo bioherbicidal activities of the compounds were evaluated in pots against 10 weeds such as Abutilon avicennae, Aeschynomene indica, Agropyron smithii, Calystegia japonica, Digitaria sanguinalis, Echinochloa crus-galli, Panicum dichotomiflorum, Solanum nigrum, Sorghum bicolor and Xanthium strumarium. Of 17 compounds tested, 6 compounds including 3-coumaranone (3CM), o-coumaric acid (OCA), coumarin (CM), protocatechuic acid ethyl ester (PAEE), scopoletin (SCP) and umbelliferone (UF) showed over 80% inhibition against L. paucicostata at the level of 125 ppm, and 3 compounds including CM, SCP and PAEE were highly inhibitive with IC50 value of 10 - 20 ppm. Hydroquinone (HQ) and PAEE were very active against C. regularis, representing the inhibition zone of >32 mm. PAEE, 3CM and HQ showed 70-80% inhibitory activity against weeds in pots. Interestingly, HQ and PAEE which were active against C. regularis were also highly phytotoxic to weeds. Our results show some plant-derived compounds including HQ and PAEE may be useful for natural bioherbicide in Korea