Feeder-free differentiation and expansion for T cells from induced pluripotent stem cells

Abstract Clinical efficacy demonstrated by chimeric antigen receptor T cell therapy call for further development that could broaden their applicability. One such direction is to develop alternate T-cell sources and T cells differentiated from pluripotent stem cells may be an ideal candidate.The present protocol provides a feeder-free and scalable method to generate T lymphocytes from induced pluripotent stem cells.