Functionalized Persistent Luminescence Nanoparticle-Based Aptasensor for Autofluorescence-free Determination of Kanamycin in Food Samples

卡那霉素 化学 适体 检出限 荧光 分析物 发光 自体荧光 色谱法 生物化学 分子生物学 材料科学 量子力学 生物 基因 物理 光电子学
作者
Beibei Wang,Xu Zhao,Lijian Chen,Cheng Yang,Xiu‐Ping Yan
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:93 (4): 2589-2595 被引量:49
标识
DOI:10.1021/acs.analchem.0c04648
摘要

Selective and sensitive determination of trace kanamycin in complex food samples is of great importance for food safety because of its high toxicity. Here, we report a sensitive and autofluorescence-free persistent luminescence (PL) aptasensor for selective, sensitive, and autofluorescence-free determination of kanamycin in food samples. The aptamer for kanamycin was first conjugated onto the surface of magnetic nanoparticles Fe3O4 to serve as the recognition unit as well as the separation element, while the PL nanoparticles ZnGa2O4:Cr (PLNPs) were functionalized with the aptamer complementary DNA (cDNA) as the PL signal. The PL aptasensor consisted of the aptamer-conjugated MNPs (MNPs-apt) and cDNA-functionalized PLNPs (PLNPs-cDNA) and combined the merits of the long-lasting luminescence of PLNPs, the magnetic separation ability of MNPs as well as the selectivity of the aptamer, offering a promising approach for autofluorescence-free determination of kanamycin in food samples. The proposed aptasensor showed excellent linearity in the range from 1 pg mL–1 to 5 ng mL–1 with a limit of detection of 0.32 pg mL–1. The precision for 11 replicate determinations of 100 pg mL–1 kanamycin was 3.1% (relative standard deviation). The developed aptasensor was applied for the determination of kanamycin in milk and honey samples with the recoveries of 95.4–106.3%. The proposed aptasensor is easily extendable to other analytes by simply replacing the aptamer, showing great potential as a universal aptasensor platform for selective, sensitive, and autofluorescence-free detection of hazardous analytes in food samples.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
Jonathan完成签到,获得积分10
1秒前
wsj发布了新的文献求助10
2秒前
3秒前
整齐小松鼠应助wsj采纳,获得10
7秒前
7秒前
8秒前
8秒前
11秒前
11秒前
11秒前
12秒前
Owen应助超速也文章采纳,获得10
13秒前
张雯思发布了新的文献求助10
15秒前
清爽尔安发布了新的文献求助10
15秒前
16秒前
孙燕应助幸福大白采纳,获得30
16秒前
香香应助研友_Zzrx6Z采纳,获得10
16秒前
18秒前
19秒前
19秒前
从容的柜子完成签到 ,获得积分10
20秒前
20秒前
木可发布了新的文献求助10
21秒前
清爽尔安完成签到,获得积分10
22秒前
Komorebi完成签到 ,获得积分10
22秒前
qqq发布了新的文献求助10
22秒前
所所应助独特乘云采纳,获得10
23秒前
24秒前
25秒前
小蘑菇应助发疯的草莓采纳,获得10
26秒前
小绵羊发布了新的文献求助10
27秒前
八卦巧克力完成签到,获得积分10
27秒前
lzw发布了新的文献求助10
28秒前
iNk应助wodetaiyangLLL采纳,获得10
31秒前
32秒前
帆帆完成签到,获得积分10
32秒前
34秒前
36秒前
future完成签到 ,获得积分10
36秒前
wwl关闭了wwl文献求助
37秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989406
求助须知:如何正确求助?哪些是违规求助? 3531522
关于积分的说明 11254187
捐赠科研通 3270174
什么是DOI,文献DOI怎么找? 1804901
邀请新用户注册赠送积分活动 882105
科研通“疑难数据库(出版商)”最低求助积分说明 809174