Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during Epididymal Transit of Sperm

精子 生物 精子细胞 精子活力 细胞生物学 鞭毛 精子发生 运动性 附睾 顶体 男科 遗传学 基因 内分泌学 医学
作者
Xiaoning Zhang,Wenwen Zhou,Peng Zhang,Fengxin Gao,Xiuling Zhao,Winnie Shum,Xuhui Zeng
出处
期刊:International Journal of Molecular Sciences [MDPI AG]
卷期号:22 (2): 652-652 被引量:17
标识
DOI:10.3390/ijms22020652
摘要

The calcium-binding protein spermatid-associated 1 (Cabs1) is a novel spermatid-specific protein. However, its function remains largely unknown. In this study, we found that a long noncoding RNA (lncRNA) transcripted from the Cabs1 gene antisense, AntiCabs1, was also exclusively expressed in spermatids. Cabs1 and AntiCabs1 knockout mice were generated separately (using Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-Cas9 methods) to investigate their functions in spermatogenesis. The genetic loss of Cabs1 did not affect testicular and epididymal development; however, male mice exhibited significantly impaired sperm tail structure and subfertility. Ultrastructural analysis revealed defects in sperm flagellar differentiation leading to an abnormal annulus and disorganization of the midpiece–principal piece junction, which may explain the high proportion of sperm with a bent tail. Interestingly, the proportion of sperm with a bent tail increased during transit in the epididymis. Furthermore, Western blot and immunofluorescence analyses showed that a genetic loss of Cabs1 decreased Septin 4 and Krt1 and increased cyclin Y-like 1 (Ccnyl1) levels compared with the wild type, suggesting that Cabs1 deficiency disturbed the expression of cytoskeleton-related proteins. By contrast, AntiCabs1−/− mice were indistinguishable from the wild type regarding testicular and epididymal development, sperm morphology, concentration and motility, and male fertility. This study demonstrates that Cabs1 is an important component of the sperm annulus essential for proper sperm tail assembly and motility.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
欣喜惜筠发布了新的文献求助10
1秒前
1秒前
无情蛋筒完成签到 ,获得积分10
3秒前
4秒前
4秒前
暴躁章鱼发布了新的文献求助10
4秒前
威武的妍完成签到,获得积分10
6秒前
7秒前
虚幻夜梦发布了新的文献求助10
7秒前
LEOhard完成签到,获得积分10
8秒前
8秒前
科研通AI2S应助欣喜惜筠采纳,获得10
9秒前
9秒前
11秒前
BJQ666发布了新的文献求助30
11秒前
12秒前
12秒前
xiaobai发布了新的文献求助10
15秒前
16秒前
mol完成签到,获得积分10
16秒前
无私的含海完成签到,获得积分10
17秒前
livrese发布了新的文献求助10
18秒前
雨中过客发布了新的文献求助30
18秒前
ding应助怡然赛君采纳,获得10
19秒前
小肥仔完成签到,获得积分10
20秒前
fengcosky发布了新的文献求助10
23秒前
Ava应助leyellows采纳,获得10
24秒前
刘老哥6发布了新的文献求助10
27秒前
CipherSage应助心行采纳,获得10
33秒前
bearinlearning完成签到,获得积分10
33秒前
大个应助奶姜采纳,获得10
35秒前
cnbhhhhh完成签到,获得积分10
35秒前
37秒前
Cpp完成签到,获得积分10
38秒前
肚皮完成签到 ,获得积分10
39秒前
39秒前
Yh_alive完成签到,获得积分10
40秒前
swh发布了新的文献求助10
41秒前
42秒前
干不了一点完成签到,获得积分10
43秒前
高分求助中
Histotechnology: A Self-Instructional Text 5th Edition 2000
Rock-Forming Minerals, Volume 3C, Sheet Silicates: Clay Minerals 2000
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
The Healthy Socialist Life in Maoist China 600
The Vladimirov Diaries [by Peter Vladimirov] 600
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3270654
求助须知:如何正确求助?哪些是违规求助? 2910067
关于积分的说明 8352062
捐赠科研通 2580504
什么是DOI,文献DOI怎么找? 1403528
科研通“疑难数据库(出版商)”最低求助积分说明 655854
邀请新用户注册赠送积分活动 635223