A two-dimensional (2D) picometer comb, a novel optical element made by picometer-differential four times exposed in two perpendicular directions, is proposed to generate the dot array projection pattern for three-dimensional (3D) shape reconstruction and other applications. Not only does a 2D picometer comb generate a stable light field distribution with extremely long depth of field and small divergence angle as a one-dimensional picometer comb, it also has new properties, such as periodicity of diffraction field in two perpendicular directions and high concentration of energy of points, which is particularly suitable for providing dot array structured light. We demonstrate that the diffraction field of a 2D picometer comb provides a solution for non-defocusing 3D reconstruction with a dot array. In fabrication of a 2D picometer comb, we can modulate the holography by changing the angle of two beams slightly, so its period can be measured at picometer accuracy. A 2D picometer comb can be made to any scale, then it can be integrated to mobile devices, such as a mobile phone, for 3D shape reconstruction. Furthermore, the concept of a 2D picometer comb would be applied to generate a picometer light field for opening the door of pico-optics in the future.