Molecular Insights into the Architecture of the Human SMC5/6 Complex

DNA 三聚体 细胞生物学 生物 生物物理学 化学 遗传学 有机化学 二聚体
作者
Marek Adamus,E. Lelkes,David Potěšil,Sri Ranjani Ganji,Peter Kolesár,Kateřina Zábrady,Zbyněk Zdráhal,Jan Paleček
出处
期刊:Journal of Molecular Biology [Elsevier BV]
卷期号:432 (13): 3820-3837 被引量:29
标识
DOI:10.1016/j.jmb.2020.04.024
摘要

A family of Structural Maintenance of Chromosome (SMC) complexes is essential for key cellular processes ensuring proper cohesion, condensation and replication. They share a common SMC-kleisin architecture allowing them to embrace DNA. In SMC5/6, the NSE1 and NSE3 KITE and NSE4 kleisin subunits form a stable subcomplex that binds DNA and regulates essential processes. In addition, NSE5 and NSE6 subunits associate with the core SMC5/6 complex and recruit it to DNA repair sites. The architecture of the SMC5/6 complex is crucial for its proper functioning, and mutations within the human SMC5/6 subunits result in severe syndromes. Therefore, we aimed to analyze interactions within the human SMC5/6 complex and determine its detailed architecture. Firstly, we analyzed different parts of SMC5/6 by crosslinking and MS/MS analysis. Our data suggested domain arrangements of hNSE1–hNSE3 and orientation of hNSE4 within the hNSE1–hNSE3–hNSE4 subcomplex. The crosslinking and electron microscopic analysis of the SMC5/6 core complex showed its rod-like architecture with juxtaposed hSMC5–hSMC6 arms. Additionally, we observed fully or partially opened hSMC5–hSMC6 shapes with the hNSE1–hNSE3–hNSE4 trimer localized in the SMC head domains. To complete mapping of the human SMC5/6 complex architecture, we analyzed positions of hNSE5-hNSE6 at the hSMC5–hSMC6 arms. We showed that hNSE6 binding to hNSE5 and the coiled-coil arm of hSMC6 is mediated by a conserved FAM178 domain, which we therefore renamed CANIN (Coiled-coil SMC6 And NSE5 INteracting) domain. Interestingly, hNSE6 bound both hSMC5 and hSMC6 arms, suggesting that hNSE6 may lock the arms and regulate the dynamics of the human SMC5/6 complex.
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