A comparative study on routine diagnostic methods in infection of Helicobacter pylori

医学 幽门螺杆菌 幽门螺杆菌感染 胃肠病学 内科学 快速尿素酶试验 胃炎 尿素呼气试验
作者
Yuanyuan Zhao,Jianming Xu,Lei Zhang,Yang Li,Hui Feng
出处
期刊:Chinese Journal of Digestive Endoscopy 卷期号:33 (6): 379-383
标识
DOI:10.3760/cma.j.issn.1007-5232.2016.06.008
摘要

Objective To investigate the efficacy of the four commonly used diagnostic methods in infection of Helicobacter pylori(H. pylori). Methods The study included 133 patients with upper gastrointestinal symptoms. Three fresh gastric biopsy were extracted from the lesser curvature of antrum when patients accepting gastroscopy, for the rapid urease test(RUT) test strip, RUT gel method, and histological examination, respectively.14C-urease breath test(14C-UBT) was carried out 1 hour after the procedure. The result of histological examination was served as the golden reference.Any two tests positive were served as the relative golden reference.The diagnostic accuracy of the four methods and the consistence with golden reference and relative golden reference was evaluated. Results The positive results were obtained in 111 cases by RUT test strip, 76 by RUT gel method, 70 by 14C-UBT, and 62 by Warthin—Starry stain. With Warthin—Starry positive as the golden reference, the finding of RUT test strip was poorly consistent with the result of Warthin—Starry(Kappa=0.295). However, the findings of RUT gel method and 14C-UBT were highly consistent with the result of Warthin—Starry(Kappa=0.672, 0.670). Because of the poor specificity of RUT test strip, any two positive tests from Warthin—Starry, RUT gel method and 14C-UBT were used as the relative golden reference to evaluate the diagnostic accuracy. The findings of Warthin—Starry and 14C-UBT were perfectly consistent with the golden reference(Kappa=0.821, 0.850), and the finding of RUT gel method was highly consistent with the golden reference(Kappa=0.763). However, the finding of RUT test strip was poorly consistent with golden reference(Kappa=0.280). When the delta cutoff value for the UBT was calculated as a standard value(DMP≥100), the area under the ROC curve was 0.932 (0.884-0.979), and the sensitivity, specificity and accuracy of 14C-UBT were 100.0%, 86.3% and 92.5%, respectively.When the delta cutoff value for the UBT was recalculated as a new value(DMP≥166), the area under the ROC curve increased to 0.949(0.906-0.992). With the golden reference as the evaluation, the sensitivity, specificity and accuracy of UBT test were 96.7%, 93.2% and 94.7%, respectively. Conclusion RUT test strip shows low accuracy; RUT gel method, UBT and histological examination have high accuracy and similar efficacy. When the delta cutoff value for the test is recalculated as DMP≥166, the accuracy of the test can be improved. Key words: Helicobacter pylori; Rapid urease test; Histology; Urease breath test
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
沉默凡梦完成签到,获得积分10
刚刚
人谷发布了新的文献求助10
1秒前
dong应助魏伯安采纳,获得10
1秒前
王哥发布了新的文献求助10
1秒前
1秒前
CQ发布了新的文献求助30
2秒前
2秒前
CC完成签到 ,获得积分10
2秒前
3秒前
罗大壮发布了新的文献求助10
3秒前
walu发布了新的文献求助10
3秒前
HHHHH完成签到,获得积分10
3秒前
4秒前
lalala发布了新的文献求助10
5秒前
pluto应助郭小胖14采纳,获得10
5秒前
5秒前
5秒前
CipherSage应助菠萝采纳,获得10
5秒前
cnm完成签到,获得积分20
6秒前
mo完成签到,获得积分10
6秒前
静俏发布了新的文献求助10
6秒前
111发布了新的文献求助10
6秒前
6秒前
传奇3应助QingSun1采纳,获得10
6秒前
小米饭完成签到,获得积分10
6秒前
量子星尘发布了新的文献求助10
7秒前
勤劳小海豚完成签到,获得积分10
8秒前
Wu关闭了Wu文献求助
8秒前
8秒前
zzk发布了新的文献求助10
8秒前
爽哥发布了新的文献求助30
8秒前
听话的不乐完成签到,获得积分10
9秒前
langjiaqi关注了科研通微信公众号
9秒前
9秒前
淡淡的襄完成签到,获得积分10
9秒前
msy发布了新的文献求助10
9秒前
天天快乐应助魅雪霓采纳,获得10
10秒前
szw发布了新的文献求助10
10秒前
wuhuanga发布了新的文献求助10
10秒前
玫瑰遇上奶油完成签到,获得积分10
10秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Picture Books with Same-sex Parented Families: Unintentional Censorship 500
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 360
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3971091
求助须知:如何正确求助?哪些是违规求助? 3515797
关于积分的说明 11179488
捐赠科研通 3250872
什么是DOI,文献DOI怎么找? 1795536
邀请新用户注册赠送积分活动 875891
科研通“疑难数据库(出版商)”最低求助积分说明 805207