化学
DNA
电极
催化作用
纳米技术
亚甲蓝
微泡
电化学
纳米复合材料
外体
适体
纳米颗粒
组合化学
生物物理学
分子生物学
材料科学
生物化学
基因
小RNA
物理化学
生物
光催化
作者
David Monchaud,Qihang Cao,Qiumei Feng
标识
DOI:10.1016/j.aca.2020.08.036
摘要
The detection of a small number of exosomes provides the possibility for early cancer diagnosis and prognosis. Here, a multi-signal amplified electrochemical sensing platform was explored for the ultrasensitive detection of tumor exosomes relying on catalytic hairpin assembly-triggered DNA walker, entropy beacon-based DNA assembly and [email protected] core-shell nanocomposites. In this work, the utilization of [email protected] nanocomposites as electrode interface effectively enhanced functional active sites and electron transfer capability. By designing a target-assisted entropy beacon-based DNA assembly, single exosome initiated the release of multiple special DNA sequences, which could be separated conveniently by magnet and then hybridize with the blocking DNA to liberate swing arm. DNA walker was activated with the assistance of catalytic hairpin assembly, introducing extensive electroactive methylene blue (MB) to electrode surface. Thus, the detection of exosomes was transferred into the measurement of the MB current, with a good liner range from 100 to 75 000 particles/μL. Furthermore, this constructed sensing system displayed acceptable reproducibility, long-term stability, favorable selectivity, and highlighting application potential in real samples.
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