Effect of MIPU1 gene interference on biological behavior of U251 cell line.

To investigate effect of MIPU1 silence on proliferation, apoptosis, migration and invasion in U251 cells.The shRNA recombinant plasmids targeting MIPU1 gene was transfected into U251 cells. Western blotting was used to identify the inhibitory efficiency at 72 h after transfection. The cell viability was measured by MTT colorimetric assay. Hoechest staining and caspase-3 activity were used to detect apoptosis. Then wound healing assay and transwell migration assay were applied to detect the migration and invasion of cells.The expression of MIPU1 protein was effectively knocked down in transfected cells (P<0.05). The cellular proliferation was obviously inhibited and apoptosis was increased in shRNA-transfected MIPU1 cells (all P<0.05). The migration and invasion ability of cells transfected with positive plasmid was lower than that in the control group (P<0.05).Down-regulation of MIPU1 can promote apoptosis while inhibit the proliferation, invasion, and migration of U251 cells.目的: 探讨特异性短发夹RNA(shRNA)干扰心肌缺血预适应上调基因1(myocardial ischemia preconditioning upregulated gene 1，MIPU1)对人脑星形细胞瘤U251细胞增殖、凋亡、迁移、侵袭能力的影响。方法: 采用瞬时转染MIPU1 shRNA干扰质粒，蛋白质印迹检测其干扰效率，MTT法检测各组细胞的增殖情况，Hoechest染色法和caspase-3活性检测细胞凋亡程度，划痕实验及Transwell实验检测细胞迁移及侵袭能力。结果: 转染MIPU1 shRNA可明显降低MIPU1蛋白的表达(P<0.05)；与对照组比较，MIPU1 shRNA组的U251细胞增殖能力下降，凋亡明显增加，细胞迁移及侵袭能力减弱(均P<0.05)。结论: MIPU1 shRNA能有效降低U251细胞中MIPU1蛋白的表达，干扰MIPU1的表达可促进U251细胞凋亡，抑制U251细胞的增殖、迁移及侵袭。.