扫描电镜
扫描离子电导显微镜
显微镜
荧光显微镜
扫描电子显微镜
扫描探针显微镜
荧光寿命成像显微镜
分辨率(逻辑)
薄层荧光显微镜
荧光
扫描共焦电子显微镜
材料科学
化学
纳米技术
分析化学(期刊)
光学
受激发射
激光器
物理
复合材料
人工智能
色谱法
计算机科学
作者
Philipp Hagemann,Astrid Gesper,Patrick Happel
出处
期刊:ACS Nano
[American Chemical Society]
日期:2018-05-23
卷期号:12 (6): 5807-5815
被引量:29
标识
DOI:10.1021/acsnano.8b01731
摘要
Correlation microscopy combining fluorescence and scanning probe or electron microscopy is limited to fixed samples due to the sample preparation and nonphysiological imaging conditions required by most probe or electron microscopy techniques. Among the few scanning probe techniques that allow imaging of living cells under physiological conditions, scanning ion conductance microscopy (SICM) has been shown to be the technique that minimizes the impact on the investigated sample. However, combinations of SICM and fluorescence microscopy suffered from the mismatch in resolution due to the limited resolution of conventional light microscopy. In the last years, the diffraction limit of light microscopy has been circumvented by various techniques, one of which is stimulated emission depletion (STED) microscopy. Here, we aimed at demonstrating the combination of STED and SICM. We show that both methods allow recording a living cellular specimen and provide a SICM and STED image of the same sample, which allowed us to correlate the membrane surface topography and the distribution of the cytoskeletal protein actin. Our proof-of-concept study exemplifies the benefit of correlating SICM with a subdiffraction fluorescence method and might form the basis for the development of a combined instrument that would allow the simultaneous recording of subdiffraction fluorescence and topography information.
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