光学
体内
临床前影像学
双光子激发显微术
材料科学
物理
生物
荧光
生物技术
作者
Cheng Jin,Ruheng Shi,Chi Liu,Lingjie Kong
出处
期刊:Chinese Optics Letters
[Shanghai Institute of Optics and Fine Mechanics]
日期:2021-01-01
卷期号:19 (12): 121701-121701
被引量:2
标识
DOI:10.3788/col202119.121701
摘要
Localized wavefront aberrations would introduce artifacts in biomedical imaging, which, however, are often neglected, as their compensations are at the cost of the field-of-view. Here, we show rarely reported local artifacts in two-photon imaging of dendrites beneath blood vessels in a mouse brain in vivo and interpret the phenomena via numerical simulations. The artifacts of divided parallel structures are found to be induced by coma and astigmatism, resulting from sample tilting and the cylinder shape of vasculatures, respectively. Different from that in single-photon microscopy, such artifacts in nonlinear microscopy show unique characteristics and should be recognized for proper interpretation of the images.
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