Optimization of the composite enzyme extraction of polysaccharide from Erythronium sibiricum bulb and its immunoregulatory activities

阿拉伯糖 糖醛酸 半乳糖 多糖 化学 鼠李糖 纤维素酶 木糖 淀粉酶 色谱法 生物化学 萃取(化学) 食品科学 发酵
作者
Yue Zhou,Shujing Yan,Shanshan Gao,Wei Guo,Xiangyun Xie,Gulibahar Kawul,Mei Wang,Yue Feng,Chunli Chen
出处
期刊:Preparative Biochemistry & Biotechnology [Taylor & Francis]
卷期号:52 (6): 681-690 被引量:5
标识
DOI:10.1080/10826068.2021.1986720
摘要

An efficient compound enzyme extraction process was developed and optimized to extract the polysaccharide from Erythronium sibiricum bulb via response surface methodology. The polysaccharide E2P was obtained. Then, the preliminary characteristics of E2P were determined via colorimetry and chromatography. Additionally, the immunoregulatory activities of E2P and another polysaccharide (ESBP, extracted using the hot water method) were compared. The optimized extraction results were as follows: temperature (54.56 °C), time (2.52 h), pH (6.53), and enzyme concentration ratio (0.5% cellulase:1.5% amylase). The yield (64.18% ± 2.91%) obtained under the aforementioned conditions was considerably higher than the yield of ESBP (37.25% ± 0.17%). The total sugar, uronic acid, starch, and protein contents of E2P were 81.77% ± 2.84%, 3.31% ± 0.45%, 3.29% ± 0.01%, and 0.24% ± 0.02%, respectively. The HPLC result suggested that the predominant monosaccharides of E2P included glucose, galactose, and arabinose, with a molar ratio of 543.2:1:1.8. The in vitro tests in RAW264.7 cells indicated that ESBP exhibited better immunomodulatory activities than E2P. In particular, ESBP can promote the proliferation, phagocytosis, and cytokine secretion abilities of cytokines, such as nitric oxide, tumor necrosis factor-α, and interleukin (IL)-1β of RAW264.6 cells. By contrast, E2P can only promote phagocytosis ability and the secretion of IL-1β.
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