纤维素酶
微晶纤维素
聚乙二醇
PEG比率
纤维素
化学
水解
色谱法
壳聚糖
核化学
化学工程
材料科学
有机化学
财务
工程类
经济
作者
Yang Wang,Chenyu Feng,Rong-Bo Guo,Yifang Ma,Yu Yuan,Yanping Liu
标识
DOI:10.1016/j.procbio.2021.02.018
摘要
A novel method of immobilizing cellulase on sodium alginate(SA)-polyethylene glycol(PEG)-Chitosan(CS) enables make the cellulase to be used repeatedly. The matrix of the immobilized cellulase was detected and characterized using Fourier transform infrared spectroscopy and scanning electron microscope. In addition, microcrystalline cellulose was hydrolyzed by the immobilized cellulase and its microstructure was further analyzed for comparison. The results showed that PEG added into SA-immobilized cellulase increased the porosity of matrix, then the hydrogen bond was formed between PEG and SA; and adding CS could form the polyelectrolyte membrane, it would reduce the disintegration of the carrier to improve the stability of the carrier. Meanwhile, the activities of immobilized cellulase and free enzyme were compared. In addition, the conditions for the SA-PEG-CS-immobilized cellulase to hydrolyze microcrystalline cellulose (MCC) were optimized by response surface method (RSM), and the interaction between independent variables was explored by analysis of variance (ANOVA). The overall yield of reducing sugar of the MCC hydrolyzed by SA-PEG-CS-immobilized cellulase was 22.68 % higher than that of the free cellulase after reusing it for 5 cycles. The strength of O3-H-O5 was enhanced and the proportion of amorphous area was decreased.
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