细胞内
多糖
菌丝体
细胞内pH值
化学
细胞外
生物化学
生物
植物
作者
Xiaoyu Wang,Yishui Wang,Wei Pan,Jinping Wang,Xiaobo Sun
出处
期刊:ACS Sustainable Chemistry & Engineering
[American Chemical Society]
日期:2021-03-03
卷期号:9 (10): 3718-3726
被引量:23
标识
DOI:10.1021/acssuschemeng.0c08160
摘要
It is difficult to quantitatively explain the effects of intracellular conditions on bioreactions in cells because direct measurements in cells are not easily achieved. In this work, a relationship between the biosynthesis of intracellular polysaccharide and intracellular pH inside fungal cells was established by quantitatively determining them using proper methods, respectively. A fluorescent ratiometric pH probe was designed and synthesized for the detection of intracellular pH by conjugating a pH-sensitive moiety and pH-insensitive carbon dots (CDs) into one system. The intracellular pH values and intracellular polysaccharide contents inside the mycelia of Pholiota adipose fungus incubated under different conditions were individually analyzed by the ratiometric pH probe and a phenol-sulfuric acid method. Thereafter, a relationship was established using the content of polysaccharide in mycelia as ordinates and intracellular pH values as abscissa. As can be seen from the relationship, intracellular pH in the range 7.10–7.25 inside Pholiota adipose mycelia is the most suitable condition for the biosynthesis of intracellular polysaccharide.
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