配对
微流控
吞吐量
生物系统
计算机科学
单细胞分析
纳米技术
细胞
化学
生物
材料科学
物理
无线
电信
量子力学
生物化学
超导电性
作者
Lei Fan,Zhangyan Guan,Tao Luo,Jifeng Ren,Raymond H. W. Lam,Dong Sun
出处
期刊:Biomicrofluidics
[American Institute of Physics]
日期:2021-09-01
卷期号:15 (5)
被引量:4
摘要
Single-cell level coculture facilitates the study of cellular interactions for uncovering unknown physiological mechanisms, which are crucial for the development of new therapies for diseases. However, efficient approaches for high-throughput deterministic pairing of single cells and traceable coculture remain lacking. In this study, we report a new microfluidic device, which combines hydrodynamic and recirculation flow captures, to achieve high-throughput and deterministic pairing of single cells in a microwell array for traceable coculture. Compared with the existing techniques, the developed device exhibits advantages with regard to pairing efficiency, throughput, determinacy, and traceability. Through repeating a two-step method, which sequentially captures single cells in a meandering channel and a microwell array, cell number and type can be easily controlled. Double and triple single-cell pairings have been demonstrated with an efficiency of 72.2% and 38.0%, respectively. Cellular engulfment using two breast cell lines is investigated on a developed microfluidic chip as a biological case study, in which the morphological characteristics and the incidence rate are analyzed. This research provides an efficient and reliable alternative for the coculture of single cells on the microfluidic platform for various biomedical applications, such as studying cellular engulfment and tumor sphere formation under single-cell pairing condition.
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