聚糖
凝集素
细胞外小泡
药物输送
化学
细胞
细胞生物学
靶向给药
微阵列
计算生物学
生物
生物化学
糖蛋白
基因
基因表达
有机化学
作者
Asako Shimoda,Risako Miura,Hiroaki Tateno,Naohiro Seo,Hiroshi Shiku,Shin‐ichi Sawada,Yoshihiro Sasaki,Kazunari Akiyoshi
标识
DOI:10.1002/smtd.202100785
摘要
Extracellular vesicles (EVs) are released by all types of mammalian cells for cell-cell communication. In this study, surface glycans on EVs are compared in terms of their cell type, size, and isolation method to examine whether EV glycan profiles by lectin microarray can be used to define EV subpopulations. Moreover, EVs are glycoengineered with four distinctive surface glycan patterns and evaluated their cellular uptake efficiencies for potential drug delivery applications. Both similarities and differences in glycan patterns are identified on EVs obtained under each experimental condition. EV size- and isolation method-dependent lectin-binding patterns are observed. Moreover, cellular uptake behaviors of EVs are affected by EV glycan profiles and acceptor cells. The in vivo biodistribution of EVs is also dependent on their glycan profile. These results suggest that EV surface glycans are a potential novel indicator of EV heterogeneity, and glycoengineering is a useful approach to regulate cell-EV interactions for biomedical applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI