化学
抗氧化剂
类黄酮
苷元
食品科学
消化(炼金术)
异鼠李素
碳酸钙-2
多酚
生物化学
DPPH
体外
糖苷
色谱法
山奈酚
有机化学
作者
Ruixue Guo,Xiaoxiao Chang,Xinbo Guo,Charles S. Brennan,Tong Li,Xiong Fu,Rui Hai Liu
出处
期刊:Food & Function
[The Royal Society of Chemistry]
日期:2017-01-01
卷期号:8 (11): 4229-4240
被引量:59
摘要
Phenolics, antioxidant and antiproliferative properties of Sea buckthorn berries were evaluated using a simulated in vitro digestion and compared with a chemical extraction method. Digested samples were subjected to antiproliferation evaluation against human liver, breast and colon cancer cells. Furthermore, the bioaccessibility of digested berries was evaluated using a Caco-2 cell culture model. Results revealed that after enzymatic digestion the phenolic compounds were quite different from the chemical extracts, more flavonoid aglycones were released, whereas less total phenolics, phenolic acids and flavonoid glycosides were detected. Although the extracellular antioxidant activity of the digesta was lower than that of extracts, the cellular antioxidant activity (CAA) and antiproliferative effects of berries were significantly enhanced by digestion. This was attributed to their higher flavonoid aglycone content and could be verified by testing individual active compounds, suggesting that the cellular uptake of samples might be improved, which was also certified by the Caco-2 cell uptake model. The digested samples showed an almost 5-fold cellular accumulative amount of isorhamnetin than pure isorhamnetin, which was attributed to the significant down regulation of the mRNA expression level of efflux transporters MRP2 and P-gp. This finding indicated that the digestion enhanced the bioaccessibility of bioactive compounds of berries.
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