Rational Design, Synthesis, Characterization, and Anti-bacterial activity of Urea Derivatives Bearing 1,2,4-triazoles as Molecular Hybrid Scaffolds

A new set of molecular hybrids, urea derivatives carrying 1,2,4-triazole as molecular hybrid scaffolds, were developed, synthesised, characterised, and assessed for potential anti-bacterial action. Triazole scaffolds are key moieties in many pharmacological compounds, and their inclusion with urea moiety makes them more valuable for biological purposes. As a result, urea derivatives containing 1H-1,2,4-triazole and 3-(methylthio)-1H-1,2,4-triazole moieties were produced by reacting carbamates of 4-amino-1,2,4-triazoles and 3-(methylthio)-4-amino-1,2,4-triazole with amines in DMF using trimethyl amine for 15 hours at ca. 60°C. 1H NMR, 13C NMR, FTIR, and HRMS techniques were used to characterise all of the produced compounds. The anti-bacterial activity of all produced derivatives was examined against Enterococcus faecalis, Pseudomonas, E. Coli, Klebsiella pneumonia, Candida albicans, Bacillus anthracis, Proteus mirabilis, and Staphylococcus aureus.