极化(电化学)
平移运动
绕固定轴旋转
牵引(地质)
激发
物理
调制(音乐)
纳米技术
光学
材料科学
化学
生物物理学
经典力学
声学
地貌学
生物
地质学
物理化学
量子力学
作者
Lingzhi Wang,Yong Hou,Tongtong Zhang,Xi Wei,Yan Zhou,Dangyuan Lei,Qiang Wei,Yuan Lin,Zhiqin Chu
出处
期刊:Nano Letters
[American Chemical Society]
日期:2022-08-10
卷期号:22 (18): 7714-7723
被引量:9
标识
DOI:10.1021/acs.nanolett.2c02232
摘要
Measuring the mechanical interplay between cells and their surrounding microenvironment is vital in cell biology and disease diagnosis. Most current methods can only capture the translational motion of fiduciary markers in the deformed matrix, but their rotational motions are normally ignored. Here, by utilizing single nitrogen-vacancy (NV) centers in nanodiamonds (NDs) as fluorescent markers, we propose a linear polarization modulation (LPM) method to monitor in-plane rotational and translational motions of the substrate caused by cell traction forces. Specifically, precise orientation measurement and localization with background suppression were achieved via optical polarization selective excitation of single NV centers with precisions of ∼0.5°/7.5 s and 2 nm/min, respectively. Additionally, we successfully applied this method to monitor the multidimensional movements of NDs attached to the vicinity of cell focal adhesions. The experimental results agreed well with our theoretical calculations, demonstrating the practicability of the NV-based LPM method in studying mechanobiology and cell-material interactions.
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