Label‐free based quantitative proteomics analysis to explore the molecular mechanism of gynecological cold coagulation and blood stasis syndrome

血瘀 医学 生物信息学 内科学 病理 生物 替代医学 中医药
作者
Ying Sun,Jian‐Zi Chu,Jingran Geng,Fengli Guan,Shuan‐Cheng Zhang,Yucong Ma,Qian‐Qian Zuo,Xiao‐Zhao Jing,Hui‐Lan Du
出处
期刊:Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology [Wiley]
卷期号:306 (12): 3033-3049 被引量:5
标识
DOI:10.1002/ar.25035
摘要

Abstract Cold coagulation and blood stasis (CCBS) syndrome is one of the common traditional Chinese medicine (TCM) syndromes of gynecological diseases. However, the molecular mechanism of CCBS syndrome is still unclear. Thus, there is a need to reveal the occurrence and regulation mechanism of CCBS syndrome, in order to provide a theoretical basis for the treatment of CCBS syndrome in gynecological diseases. The plasma proteins in primary dysmenorrhea (PD) patients with CCBS syndrome, endometriosis (EMS) patients with CCBS syndrome, and healthy women were screened using Label‐free quantitative proteomics. Based on the TCM theory of “same TCM syndrome in different diseases,” the differentially expressed proteins (DEPs) identified in each group were subjected to intersection mapping to obtain common DEPs in CCBS syndrome. The DEPs of gynecological CCBS syndrome in the intersection part were again cross‐mapped with the DEPs of gynecological CCBS syndrome obtained by the research group according to the TCM theory of “different TCM syndromes in same disease” theory in the early stage, so as to obtain the DEPs of gynecological CCBS syndrome that were shared by the two parts. The common DEPs were subjected to bioinformatics analysis, and were verified by enzyme‐linked immunosorbent assay (ELISA). A total of 67 common DEPs were identified in CCBS syndrome, of which 33 DEPs were upregulated and 34 DEPs were downregulated. The functional classification of DEPs involved in metabolic process, energy production and conversion, immune system process, antioxidant activity, response to stimulus, and biological adhesion. The subcellular location mainly located in the cytoplasm, nucleus, and extracellular. Gene ontology (GO) enrichment analysis showed that the upregulated DEPs mainly concentrated in lipid transport, cell migration, and inflammatory reaction, and the downregulated DEPs mostly related to cell junction, metabolism, and energy response. Protein domain enrichment analysis and clustering analysis revealed that the DEPs mainly related to cell proliferation and differentiation, cell morphology, metabolism, and immunity. The Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis clustering analysis showed that the upregulated DEPs were involved in inflammation and oxidative damage, while the downregulated DEPs were involved in inflammation, cell adhesion, cell apoptosis, and metabolism. The results of ELISA showed significantly increased levels of Cell surface glycoprotein MUC18 (MCAM) and Apolipoprotein C1 (APOC1), and significantly decreased levels of Vasodilator‐stimulated phosphoprotein (VASP), Fatty acid‐binding protein 5 (FABP5), and Vinculin (VCL) in patients with CCBS syndrome compared with healthy women. We speculated that cold evil may affect the immune process, inflammatory response, metabolic process, energy production and conversion, oxidative damage, endothelial cell dysfunction, and other differential proteins expression to cause CCBS syndrome in gynecological diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
2秒前
潇湘雪月发布了新的文献求助10
3秒前
3秒前
3秒前
4秒前
感动黄豆发布了新的文献求助10
4秒前
hhhblabla应助东方红采纳,获得10
6秒前
Poker应助sb采纳,获得10
7秒前
Ginger发布了新的文献求助10
7秒前
吃骨头的猫完成签到,获得积分10
7秒前
小李完成签到,获得积分10
7秒前
7秒前
8秒前
明芬发布了新的文献求助30
10秒前
10秒前
Smile完成签到,获得积分10
10秒前
Chaoe完成签到,获得积分10
13秒前
建国发布了新的文献求助10
14秒前
闪闪w发布了新的文献求助10
17秒前
淡烟流水完成签到,获得积分10
17秒前
俏皮芷蕊完成签到,获得积分10
18秒前
完美世界应助忐忑的阑香采纳,获得10
18秒前
华仔应助兴奋千兰采纳,获得10
23秒前
Ginger完成签到,获得积分10
24秒前
潇湘雪月发布了新的文献求助10
27秒前
科研通AI5应助科研通管家采纳,获得10
29秒前
29秒前
佳琳有乐完成签到,获得积分10
29秒前
29秒前
小蘑菇应助科研通管家采纳,获得10
29秒前
大模型应助科研通管家采纳,获得10
29秒前
CHAosLoopy应助科研通管家采纳,获得10
30秒前
桐桐应助科研通管家采纳,获得10
30秒前
今后应助cccyq采纳,获得10
30秒前
烟花应助科研通管家采纳,获得30
30秒前
情怀应助科研通管家采纳,获得10
30秒前
30秒前
隐形曼青应助科研通管家采纳,获得10
30秒前
在水一方应助科研通管家采纳,获得30
30秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989115
求助须知:如何正确求助?哪些是违规求助? 3531367
关于积分的说明 11253688
捐赠科研通 3269986
什么是DOI,文献DOI怎么找? 1804868
邀请新用户注册赠送积分活动 882078
科研通“疑难数据库(出版商)”最低求助积分说明 809105