Label‐free based quantitative proteomics analysis to explore the molecular mechanism of gynecological cold coagulation and blood stasis syndrome

血瘀 医学 生物信息学 内科学 病理 生物 替代医学 中医药
作者
Ying Sun,Jian‐Zi Chu,Jingran Geng,Fengli Guan,Shuan‐Cheng Zhang,Yucong Ma,Qian‐Qian Zuo,Xiao‐Zhao Jing,Hui‐Lan Du
出处
期刊:Anatomical Record-advances in Integrative Anatomy and Evolutionary Biology [Wiley]
卷期号:306 (12): 3033-3049 被引量:5
标识
DOI:10.1002/ar.25035
摘要

Abstract Cold coagulation and blood stasis (CCBS) syndrome is one of the common traditional Chinese medicine (TCM) syndromes of gynecological diseases. However, the molecular mechanism of CCBS syndrome is still unclear. Thus, there is a need to reveal the occurrence and regulation mechanism of CCBS syndrome, in order to provide a theoretical basis for the treatment of CCBS syndrome in gynecological diseases. The plasma proteins in primary dysmenorrhea (PD) patients with CCBS syndrome, endometriosis (EMS) patients with CCBS syndrome, and healthy women were screened using Label‐free quantitative proteomics. Based on the TCM theory of “same TCM syndrome in different diseases,” the differentially expressed proteins (DEPs) identified in each group were subjected to intersection mapping to obtain common DEPs in CCBS syndrome. The DEPs of gynecological CCBS syndrome in the intersection part were again cross‐mapped with the DEPs of gynecological CCBS syndrome obtained by the research group according to the TCM theory of “different TCM syndromes in same disease” theory in the early stage, so as to obtain the DEPs of gynecological CCBS syndrome that were shared by the two parts. The common DEPs were subjected to bioinformatics analysis, and were verified by enzyme‐linked immunosorbent assay (ELISA). A total of 67 common DEPs were identified in CCBS syndrome, of which 33 DEPs were upregulated and 34 DEPs were downregulated. The functional classification of DEPs involved in metabolic process, energy production and conversion, immune system process, antioxidant activity, response to stimulus, and biological adhesion. The subcellular location mainly located in the cytoplasm, nucleus, and extracellular. Gene ontology (GO) enrichment analysis showed that the upregulated DEPs mainly concentrated in lipid transport, cell migration, and inflammatory reaction, and the downregulated DEPs mostly related to cell junction, metabolism, and energy response. Protein domain enrichment analysis and clustering analysis revealed that the DEPs mainly related to cell proliferation and differentiation, cell morphology, metabolism, and immunity. The Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis clustering analysis showed that the upregulated DEPs were involved in inflammation and oxidative damage, while the downregulated DEPs were involved in inflammation, cell adhesion, cell apoptosis, and metabolism. The results of ELISA showed significantly increased levels of Cell surface glycoprotein MUC18 (MCAM) and Apolipoprotein C1 (APOC1), and significantly decreased levels of Vasodilator‐stimulated phosphoprotein (VASP), Fatty acid‐binding protein 5 (FABP5), and Vinculin (VCL) in patients with CCBS syndrome compared with healthy women. We speculated that cold evil may affect the immune process, inflammatory response, metabolic process, energy production and conversion, oxidative damage, endothelial cell dysfunction, and other differential proteins expression to cause CCBS syndrome in gynecological diseases.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
蒸馏水完成签到,获得积分10
1秒前
陈某人发布了新的文献求助10
1秒前
yinhe完成签到,获得积分10
1秒前
husy完成签到,获得积分10
2秒前
2秒前
戌博完成签到,获得积分10
3秒前
竹子发布了新的文献求助10
4秒前
彭于晏应助cb采纳,获得10
4秒前
葫芦娃完成签到,获得积分10
5秒前
Jasper应助iShine采纳,获得10
5秒前
lelele发布了新的文献求助10
6秒前
6秒前
7秒前
dongdoctor完成签到 ,获得积分10
7秒前
魏莱发布了新的文献求助10
7秒前
Ulrica完成签到,获得积分10
7秒前
CipherSage应助包容追命采纳,获得10
7秒前
owldan完成签到 ,获得积分10
9秒前
一直向前完成签到,获得积分10
9秒前
舒服的映安完成签到 ,获得积分10
9秒前
lmh011115发布了新的文献求助10
10秒前
11秒前
一直向前发布了新的文献求助10
11秒前
End完成签到 ,获得积分10
12秒前
沉静的红酒完成签到,获得积分10
13秒前
yzxzdm完成签到 ,获得积分10
13秒前
Yara.H完成签到 ,获得积分10
13秒前
Meng完成签到,获得积分10
14秒前
16秒前
量子星尘发布了新的文献求助10
18秒前
包容追命发布了新的文献求助10
20秒前
20秒前
梦鱼完成签到,获得积分10
21秒前
小林不熬夜完成签到,获得积分10
21秒前
玛卡巴卡完成签到,获得积分10
22秒前
希尔伯特发布了新的文献求助10
24秒前
Jasper应助dailyyang采纳,获得10
24秒前
冬凌草完成签到 ,获得积分10
24秒前
阿若完成签到,获得积分10
24秒前
英姑应助单纯冰棍采纳,获得10
24秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
Research on Disturbance Rejection Control Algorithm for Aerial Operation Robots 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038446
求助须知:如何正确求助?哪些是违规求助? 3576149
关于积分的说明 11374627
捐赠科研通 3305875
什么是DOI,文献DOI怎么找? 1819354
邀请新用户注册赠送积分活动 892680
科研通“疑难数据库(出版商)”最低求助积分说明 815048