Adenovirus represent the most common cause of respiratory system disorders and antibody detection can speed up and improve the sensitivity of hAdV diagnosis. Method: This study includes 90 patient who suffering from respiratory symptoms , common colds , or diarrhea with high temperature their ages range from newborn to elderly which collected from Fallujah Maternity and Children's Hospital and teaching hospital in Fallujah in Anbar governorate, for the period from November to May 1-11-2021 to 1-5-2022. Adenovirus was detected by ELISA Test for serum. Results: Out of total 90 serum sample there were 22(24.4%), 68(75.6%) Negative and positive result respectively, from IgG and 87(96.7%) ,3(3.3%) Ne gative and positive result respectively from IgM Result. Conclusion: The presence of positive IgM in ELISA cannot be relied upon as a tool for diagnosing early or acute infections with the virus.