丙二醛
细胞毒性T细胞
谷胱甘肽
细胞凋亡
氧化应激
活性氧
CYP2E1
毒性
活力测定
化学
分子生物学
药理学
细胞生物学
生物化学
生物
细胞色素P450
酶
体外
有机化学
作者
Ameer Ali Imarah,Majid S. Jabir,Ali Hassan Abood,Ghassan M. Sulaiman,Salim Albukhaty,Hamdoon A. Mohammed,Riaz A. Khan,Hayder M Al-Kuraishy,Ali I. Al‐Gareeb,Waleed Khalid Al‐Azzawi,Mazin A.A. Najm,Sabrean Farhan Jawad
出处
期刊:Nanomedicine
日期:2023-05-01
卷期号:18 (11): 875-887
被引量:2
标识
DOI:10.2217/nnm-2023-0129
摘要
Aim: The cytotoxic effects of graphene oxide nanoparticles (GONPs) using MTT assays, observance of apoptotic markers, and oxidative stress were outlined. Materials & methods: Rat embryonic fibroblasts (REFs) and human epithelial breast cells (HBLs) were used at 250, 500 and 750 μg/ml concentrations. Results: Significant cytotoxic and apoptotic effects were observed. Analyses of CYP2E1 and malondialdehyde concentrations in REF and HBL-100 cell lines after exposing to GONPs confirmed the nanomaterials toxicity. However, the glutathione levels in REF and HBL-100 cell lines showed a substantial reduction compared with the control. The cytochrome CYP2E1, glutathione, malondialdehyde and caspase-3 alterations provided a plausible interlinked relationship. Conclusion: The study confirmed the GONPs cytotoxic effects on REF and HBL-100 cell lines. The outcome suggested caution in wide-spread applications of GONPs, which could have implications for occupational health also.
科研通智能强力驱动
Strongly Powered by AbleSci AI