间充质干细胞
衰老
间质细胞
生物
干细胞
癌症研究
细胞生物学
免疫学
作者
Giuseppe Fusi,Michaël Constantinides,Christina Fissoun,Lydiane Pichard,Yves‐Marie Pers,Rosanna Ferreira-Lopez,Véronique Pantesco,Christophe Poulet,Olivier Malaise,Dominique de Seny,Jean-Marc Lemaı̂tre,Christian Jørgensen,Jean‐Marc Brondello
出处
期刊:Biomedicines
[MDPI AG]
日期:2023-07-14
卷期号:11 (7): 1994-1994
被引量:1
标识
DOI:10.3390/biomedicines11071994
摘要
Senescent cells promote progressive tissue degeneration through the establishment of a combined inflammatory and trophic microenvironment. The cellular senescence state has therefore emerged as a central driving mechanism of numerous age-related diseases, including osteoarthritis (OA), the most common rheumatic disease. Senescence hallmarks are detectable in chondrocytes, synoviocytes and sub-chondral bone cells. This study investigates how the senescence-driven microenvironment could impact the cell fate of resident osteoarticular mesenchymal stromal/stem cells (MSCs) that are hence contributing to OA disease progression. For that purpose, we performed a comparative gene expression analysis of MSCs isolated from healthy donors that were in vitro chronically exposed either to interferon-gamma (IFN-γ) or Transforming Growth Factor beta 1 (TGFβ1), two archetypical factors produced by senescent cells. Both treatments reduced MSC self-renewal capacities by upregulating different senescence-driven cycle-dependent kinase inhibitors. Furthermore, a common set of differentially expressed genes was identified in both treated MSCs that was also found enriched in MSCs isolated from OA patients. These findings highlight an imprinting of OA MSCs by the senescent joint microenvironment that changes their matrisome gene expression. Altogether, this research gives new insights into OA etiology and points to new innovative therapeutic opportunities to treat OA patients.
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