材料科学
明胶
形态学(生物学)
生物物理学
共价键
弹性体
纳米技术
硅酮
组织工程
生物材料
细胞
生物医学工程
复合材料
化学
医学
生物化学
遗传学
有机化学
生物
作者
Kun Fang,S.H. Mueller,Mitsuru Ueda,Yasuhiro Nakagawa,Katsuko Furukawa,Takashi Ushida,Toshiyuki Ikoma,Yoshihiro Ito
摘要
Fibroblasts geometrically confined by photo-immobilized gelatin micropatterns were subjected to cyclic stretch on the silicone elastomer. By using covalently micropatterned surfaces, the cell morphologies such as cell area and length were quantitatively investigated under a cyclic stretch for 20 hours. The mechanical forces did not affect the cell growth but significantly altered the cellular morphology on both non-patterned and micropatterned surfaces. It was found that cells on non-patterns showed increasing cell length and decreasing cell area under the stretch. The width of the strip micropatterns provided a different extent of contact guidance for fibroblasts. The highly extended cells on the 10 μm pattern under static conditions would perform a contraction behavior once treated by cyclic stretch. In contrast, cells with a low extension on the 2 μm pattern kept elongating according to the micropattern under the cyclic stretch. The vertical stretch induced an increase in cell area and length more than the parallel stretch in both the 10 μm and 2 μm patterns. These results provided new insights into cell behaviors under geometrical confinement in a dynamic biomechanical environment and may guide biomaterial design for tissue engineering in the future.
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