生物
诱导多能干细胞
谱系(遗传)
运行x1
造血
干细胞
髓样
谱系标记
祖细胞
转录组
免疫学
细胞生物学
遗传学
胚胎干细胞
基因
基因表达
作者
Huan Peng,Yunqing Lin,Fangxiao Hu,Cui Lv,Bingyan Wu,Qitong Weng,Lijuan Liu,Chengxiang Xia,Xiaofei Liu,Yalan Zhao,Qi Zhang,Yang Geng,Mengyun Zhang,Jinyong Wang
标识
DOI:10.1016/j.stemcr.2023.01.009
摘要
Summary
Regenerating prolonged multi-lineage hematopoiesis from pluripotent stem cells (PSCs), an unlimited cell source, is a crucial aim of regenerative hematology. In this study, we used a gene-edited PSC line and revealed that simultaneous expression of three transcription factors, Runx1, Hoxa9, and Hoxa10, drove the robust emergence of induced hematopoietic progenitor cells (iHPCs). The iHPCs engrafted successfully in wild-type animals and repopulated abundant and complete myeloid-, B-, and T-lineage mature cells. The generative multi-lineage hematopoiesis distributed normally in multiple organs, persisted over 6 months, and eventually declined over time with no leukemogenesis. Transcriptome characterization of generative myeloid, B, and T cells at the single-cell resolution further projected their identities to natural cell counterparts. Thus, we provide evidence that co-expression of exogenous Runx1, Hoxa9, and Hoxa10 simultaneously leads to long-term reconstitution of myeloid, B, and T lineages using PSC-derived iHPCs as the cell source.
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