KLF2
癌症研究
信号转导
污渍
生物
细胞生长
免疫组织化学
转录因子
免疫学
细胞生物学
基因
遗传学
作者
CHUNG-YI LIU,TZU-HSUAN CHANG,CHIN-HSUAN HSIEH,YING-HSU CHANG,JACOB SEE-TONG PANG,Cheng-Keng Chuang
出处
期刊:Anticancer Research
[Anticancer Research USA Inc.]
日期:2022-10-01
卷期号:42 (10): 4753-4762
被引量:1
标识
DOI:10.21873/anticanres.15980
摘要
Abstract
Background/Aim: The transcription factor Kruppel-like factor 2 (KLF2) is thought to act as a tumor suppressor. However, its expression and function in renal angiomyolipomas (AMLs) remains unclear. This study aimed to investigate the expression and function of KLF2 in AML cells. Materials and Methods: KLF2 was detected in AML tissues by immunohistochemistry and quantitative real-time polymerase chain reaction. The associations between KLF2 expression levels and clinicopathological features of patients with AMLs were analyzed. To explore its function in AMLs, KLF2 was over-expressed, and cell proliferation was assessed using cell counting kit-8 assay. Through Gene set enrichment analysis (GSEA) of RNA sequencing data, the signaling pathways regulated by KLF2 were predicted. The KLF2-regulated signaling pathway was validated by western blotting. Results: KLF2 expression was dramatically suppressed in clinical samples of patients with AMLs. Low KLF2 expression was significantly associated with a larger tumor size and higher incidence of tumor hemorrhage (p=0.008 and p=0.009, respectively). In addition, KLF2 overexpression markedly inhibited SV7 and UMB cell survival and proliferation. GSEA and western blotting analysis revealed that KLF2 down-regulated the IL-6/JAK/STAT3 signaling pathway. Conclusion: Collectively, KLF2 mediated AML cell growth by regulating the IL-6/JAK/STAT3 signaling pathway. These results indicate that KLF2 plays an important role in AML progression and provide novel insights into diagnostic and therapeutic biomarkers for AMLs.
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