生物
细胞生物学
有丝分裂
有丝分裂出口
核板
细胞核
细胞周期
细胞周期蛋白依赖激酶1
拉明
主轴装置
细胞质
核心
核蛋白
细胞
遗传学
细胞分裂
转录因子
基因
作者
Margarida Dantas,Andreia Oliveira,Paulo Aguiar,Hélder Maiato,Jorge G. Ferreira
标识
DOI:10.1083/jcb.202205051
摘要
As cells prepare to divide, they must ensure that enough space is available to assemble the mitotic machinery without perturbing tissue homeostasis. To do so, cells undergo a series of biochemical reactions regulated by cyclin B1-CDK1 that trigger cytoskeletal reorganization and ensure the coordination of cytoplasmic and nuclear events. Along with the biochemical events that control mitotic entry, mechanical forces have recently emerged as important players in cell-cycle regulation. However, the exact link between mechanical forces and the biochemical pathways that control mitotic progression remains unknown. Here, we identify a tension-dependent signal on the nucleus that sets the time for nuclear envelope permeabilization (NEP) and mitotic entry. This signal relies on actomyosin contractility, which unfolds the nucleus during the G2-M transition, activating the stretch-sensitive cPLA2 on the nuclear envelope and regulating the nuclear translocation of cyclin B1. Our data demonstrate how nuclear tension during the G2-M transition contributes to timely and efficient mitotic spindle assembly and prevents chromosomal instability.
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